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Test D: a simple test that has a significant effect on the Staphylococcus aureus macrolide-lincosamide-streptograminB resistance pattern.Since clindamycin and streptogrin are among the few drugs of choice in the treatment of methicillin-resistant Staphylococcus aureus (MRSA) infections, knowledge of resistance to these antibiotics is essential.Since clindamycin and streptogrin are among the few drugs of choice in the treatment of methicillin-resistant Staphylococcus aureus (MRSA) infections, knowledge of resistance to these antibiotics is essential.Since clindamycin and streptogrin are among the few drugs of choice in the treatment of methicillin-resistant Staphylococcus aureus (MRSA) infections, knowledge of resistance to these antibiotics is essential.Growth of the organism up to the edges of the disc, flattening of the area of clindamycin (D test positive) near the disc of erythromycin (resistant) and exposed to both antibiotics indicate that the organism has constitutive MLSB (CMLSB), inducible MLSB (IMLSB) and no resistance respectively.


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a summary
Test D: a simple test that has a significant effect on the Staphylococcus aureus macrolide-lincosamide-streptograminB resistance pattern.


The D test is a simple disk spread test that gives high throughput results. It is used to study streptogramin macrolide lincosamide (MLSB) resistance, both constitutive and inducible as well as macrolide streptogramin (MSB) resistance in Staphylococcus aureus. In this test, tablets of erythromycin (a macrolide) and clindamycin (a lincosamide derivative) are placed next to each other on top of Mueller-Hinton agar medium inoculated with the test organism. Growth of the organism up to the edges of the disc, flattening of the area of ​​clindamycin (D test positive) near the disc of erythromycin (resistant) and exposed to both antibiotics indicate that the organism has constitutive MLSB (CMLSB), inducible MLSB (IMLSB) and no resistance respectively. Additional, the organism is susceptible to clindamycin without any flattening of the area (negative D-test) near the clindamycin disc (resistant) indicates that the organism has macrolide streptograin (MSB) resistance. The test is performed in the same MHA panel as the antibiotic susceptibility test, with the discs placed next to each other while maintaining distance. Since clindamycin and streptogrin are among the few drugs of choice in the treatment of methicillin-resistant Staphylococcus aureus (MRSA) infections, knowledge of resistance to these antibiotics is essential. Bearing in mind that the discs are placed next to each other while maintaining the distance. Since clindamycin and streptogrin are among the few drugs of choice in the treatment of methicillin-resistant Staphylococcus aureus (MRSA) infections, knowledge of resistance to these antibiotics is essential. Bearing in mind that the discs are placed next to each other while maintaining the distance. Since clindamycin and streptogrin are among the few drugs of choice in the treatment of methicillin-resistant Staphylococcus aureus (MRSA) infections, knowledge of resistance to these antibiotics is essential.


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