Lakhasly

ABSTRACT: Bacterial halo blight, caused by Pseudomonas syringae p v. garcae, is an important disease of coffee crop occurring in Brazil and other countries.With a few exceptions, strains isolated from 1997 to 2011, grouped mainly by their region of origin, were predominantly isolated from higher altitude regions, above 800 m. This probably occurred because the climatic conditions that prevail in these regions, characterized by milder temperatures and regular rainfall, are favorable for the coffee crop and for the production of high quality coffee beverage, but can be also favorable to bacterial halo blight.The Kenyan *Corresponding author: [email protected] Received: Jul 6, 2016 - Accepted: Feb. 28, 2017 96 Bragantia, Campinas, v. 77, n. 1, p.96-106, 2018 strains grouped separately from the Brazilian strains with ERIC-PCR and the combination of ERIC- and REP-PCR.

ABSTRACT: Bacterial halo blight, caused by Pseudomonas syringae p v. garcae, is an important disease of coffee crop occurring in Brazil and other countries. In recent years, outbreaks of this disease have damaged several coffee crops in Brazil. Aggressiveness and genetic diversity of 25 strains of P. s . p v. garcae, obtained between the years 1958 and 2011, in 23 cities of São Paulo and Minas Gerais states, as well as three strains from Kenya were evaluated in this study. The strains were inoculated on coffee seedlings cultivar Mundo Novo, and their genetic diversity was evaluated by ERIC-PCR, REP-PCR, and their combination. All the strains were pathogenic to the coffee seedlings; the results of pathogenicity tests, in both experiments, could be divided in four aggressiviness classes (highly aggressive; aggressive; moderately aggressive and less aggressive). The Kenyan *Corresponding author: [email protected] Received: Jul 6, 2016 – Accepted: Feb. 28, 2017
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Bragantia, Campinas, v. 77, n. 1, p.96-106, 2018
strains grouped separately from the Brazilian strains with ERIC-PCR and the combination of ERIC- and REP-PCR. The Brazilian strains could be grouped in two sub-clusters, the first including the older strains, obtained from 1958 to 1978, and the other comprising the remaining strains. With a few exceptions, strains isolated from 1997 to 2011, grouped mainly by their region of origin, were predominantly isolated from higher altitude regions, above 800 m. This probably occurred because the climatic conditions that prevail in these regions, characterized by milder temperatures and regular rainfall, are favorable for the coffee crop and for the production of high quality coffee beverage, but can be also favorable to bacterial halo blight.