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The slide observed showed that the bacteria was stained blue with very light tints of
pink.The Gram staining method is a technique that was invented by a Danish bacteriologist
Hans Christian Joachim Gram in 1884 to classify bacteria into two major classes, that is gram
positive bacteria and gram negative bacteria.Klebsiella aerogenes and
Escherichia coli are gram negative bacteria.
The slide observed showed that the bacteria was stained blue with very light tints of
pink.
The Gram staining method is a technique that was invented by a Danish bacteriologist
Hans Christian Joachim Gram in 1884 to classify bacteria into two major classes, that is gram
positive bacteria and gram negative bacteria. This technique is still widely used until today.
Gram positive bacteria are stained dark blue or violet when observed under the
microscope whereas gram negative bacteria are stained pink in colour. Gram negative bacteria
are unable to retain the crystal violet stain, thus taking in the colour of the counter stain,
safranine (red). Klebsiella aerogenes and
Escherichia coli are gram negative bacteria. This is because they have a thin layer of peptidoglycan that causes them to lose the blue colour of the crystal
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