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Polyelectrolyte precipitation Precipitation methods are usually employed for processing large quantities of feed material proteins, offering modest enrichment and high specificity at a low cost early step in the purification process.Polyelectrolyte precipitation has been identified as a feasible method for an initial, non chromatographic purification step.Precipitation processes can be scaled-up generally for commercial and industrial operations.
Polyelectrolyte precipitation
Precipitation methods are usually employed for processing
large quantities of feed material proteins, offering modest
enrichment and high specificity at a low cost early step in the
purification process. Including a precipitation step early in
the purification process can significantly decrease the
number of steps needed in purification process by removing
large amounts of impurities and concentrating the product
feed. Precipitation processes can be scaled-up generally for
commercial and industrial operations. Some common
precipitants are acids or bases (isoelectric precipitation), salts
(‘salting out’ with ammonium sulfate), organic solvents,
polyelectrolytes, protein-binding dyes, and macroligands.
“Selective” precipitation methods such as polyelectrolyte
precipitation, protein-binding dyes, and macroligands offer
better purification than “non-selective” methods like
isoelectric precipitation, ammonium sulfate precipitation, or
organic solvents precipitations. Polyelectrolyte precipitation
has been identified as a feasible method for an initial, non
chromatographic purification step.
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