Lakhasly

3.2.5.The advantage of using ATPS technique is that it reduces the number of initial downstream steps and clarification, concentration, and partial purification can be integrated in one unit [29].Three Phase partitioning Three phase partitioning (TPP) is one of the recent methods, which has been developed for separating proteins into a midlayer and has been successfully employed for purification of a number of proteins and enzymes.This polymer-salt system results in higher selectivity in protein partitioning, resulting in an by the pH of the elution buffer.Three phase partitioning is an emerging purification method, which is a simple but elegant non chromatographic process for purification and concentration of proteins.Aqueous two phase systems (ATPS) Aqueous two phase systems (ATPS) offer an attractive alternative meeting present day requirements as well as the criteria for industrially compatible procedures.3.2.8.

3.2.5. Aqueous two phase systems (ATPS)
Aqueous two phase systems (ATPS) offer an attractive
alternative meeting present day requirements as well as the
criteria for industrially compatible procedures. This method
is increasingly receiving importance in protein purification
industry. The advantage of using ATPS technique is that it
reduces the number of initial downstream steps and
clarification, concentration, and partial purification can be
integrated in one unit
[29]. Scaling-up processes based on
aqueous two phase systems are simple. An aqueous two-
phase system is an aqueous, liquid–liquid, biphasic system
which is obtained either by mixing of aqueous solution of
two polymers, or a polymer and a salt. Generally, the former
is comprised of PEG and polymers like dextran, starch,
polyvinyl alcohol, etc. This polymer-salt system results in
higher selectivity in protein partitioning, resulting in an
by the pH of the elution buffer.
3.2.8. Three Phase partitioning
Three phase partitioning (TPP) is one of the recent methods,
which has been developed for separating proteins into a
midlayer and has been successfully employed for purification
of a number of proteins and enzymes. Three phase
partitioning is an emerging purification method, which is a
simple but elegant non chromatographic process for
purification and concentration of proteins. It is easily
scalable and can be used directly with crude suspensions. It
can handle gram quantities of crude inputs in relatively small
volumes. It was initially used as a downstream method for
isolating the enzymes cellulases
[27]. The schematic
representation of TPP is shown in Figure 8.