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Method validation for determination of OTA
The analytical method was validated by the evaluation of linearity, limits of detection (LOD) and quantification (LOQ), precision and trueness based on recovery stud ies for three matrix, wheat grain, fine semolina and fiour.regression analysis and linearity was expressed as a coefficient of determination (R^{2}) The statistical param- eters were calculated by least-square regression and LOD and LOQ were considered as 3 x S/N ratio and 10\times S/N ratio, respectively.The precision was expressed as %RSD of peak areas.
Method validation for determination of OTA
The analytical method was validated by the evaluation of linearity, limits of detection (LOD) and quantification (LOQ), precision and trueness based on recovery stud ies for three matrix, wheat grain, fine semolina and fiour. The linearity of the method was assessed by ana- lyzing OTA concentrations in the range 1-50 µg/kg in triplicate. The data for peak area were treated by linear. regression analysis and linearity was expressed as a coefficient of determination (R^{2}) The statistical param- eters were calculated by least-square regression and LOD and LOQ were considered as 3 x S/N ratio and 10\times S/N ratio, respectively. The precision and the recoveries of the method were evaluated by applica tion of the whole procedure to six samples (experi- mental replicates) spiked at final concentration of 10 µg/kg. The precision was expressed as %RSD of peak areas. Recoveries were calculated as (signal of a spiked sample/signal of a spiked extract) x 100. The fortified samples were extracted, cleaned up and ana- lyzed by HPLC-FLD as described previously
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