Lakhasly

".+,.,ii .i,"a*.,11 1{+ar 6^ a--:"^ J;.-Ldi^ .3 a+= o-r,jJl t )trl1 oLCl rA Ltlt cls c :+*"f rsjl a.-",,iYl a-l,rj Cr .+ Lilj]l c.rt-Lt - ll ";.+ .rJc ,-irJiill ,,,t1 ..{/ ( t !:u't r ,1 : Primary culture qlsyr t:l;^ll * 4 - f,/ '"'-i lAJusl J e-Jl er" !)l-i.ll J.-s +J-s ,rS Jl +l':jl o].!1 Primary cu|{u1s G-r,i .:+l uE ,rk ) il L \ii",Jl 4JsL11 a=.l ,,JI Js +Ul l).=il ,-?i:: el Jl a-i) ai+ .jr15 ,",-r'-l l+),tJl 9l .,-:43 cOnfluent stage c .-,?! L Jl J.:: !)\ilt Jd .1l gl ,(g-p::l t-!!tl d subculture l+)iAll Li : rJ.-A;:!cl cll. cell passage .r 1I"Jl .)A .,lc ?.IL .J Ili JIJ,"iYl ,1- "S;:l i+.:+ &,rjAl-Jl .,J- *-;;L : Cell tine r+)till.Li ,t,3/ cell line !)i li .+ primary culture ql-,Yl t-ll:Jl ,.--,: ,11):JJ subculture ql^c J3t :-r finite Err- r EL>>' i;;r rili U-:Yl t-lly.ll d aiii.j:^il $Jlllt= .:,i .subclone i+rj il+.,i _rl r JAil J' +J1Jt oJi.ill oi: !}AJl g+.-j passage !)liJt J"-i Ajl^C JIJ,^:-L . iife span L^1Jl lr)-ill c.o5+i ,P ao'r^ !-Bl^ cJA"- -, jlr.-r jlJA li j^.r 4iE e:-ir 1". ;sl-..Jl r al.,aty1 . cell population A : Cell strain L.JEi a? * 3 -- C" .c>l o+-rh ,i ;t..,i.,')l a1"-1:>> crl J= !A 4r jr ; la)i .'Liul .;^ 3,Hl +9lJlJ.=lf 3o ,i ;:=1, {iJ"S ,a: ;i tpti)t "l+l uS"J !}All "iA .5-a Jelu {!" .:l.;^^ _, i:i.i.o ,.ti .+ t* _r !il.S 1*)lill (?tS ljt Homogeneous population L-,iEJ!

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{æT.1'J1rÇ4-'Li , Tbtc&
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I TiSSue CultUre a-;ytâ-l;-, eX"..-^ §j,_r ilL ;l C
-: ksl-,>-l é.t r,ltr;t \sJlrJ= 6+r él rr Çl.iJt J-Â .,ji (!>itt bl:sri) a-.oyt Ll;j
It+Yl 4-ü)E g;1-»
"s
L ri;. ot:rVt À.1_,, gj^.i: l.;;l , ;r".rll â*:)l* 4*'l-- irq# .+ ÇL;l
$ 4r;rJjJl !)Âll 1..lci " organ expiants ,sJt .§Jl j:b çt-;cyl gllj;t ;l 3D culture
kot-:it i * ,1-é§!l LÈ;il 4iF" i.§:!ts*" :l 4*+-,r,1 ,j;}+ 4Lt - r e.,* ar âllj^ !>i é
LÈ;Jl .cell strain lris Âl)i- :l cell line !)- -ti 6 rêi.i^ !)\À .;é _,t r"jll4Dt" 4+
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4+J+-ll Q§Ll,* r!ili ob i;rl-§ 3 a+= o-r,jJl t )trl1 oLCl rÀ Ltlt cls ç
:+*"f rsjl â.-",,iYl a-l,rj Cr .+ Lilj]l c.rt-Lt - ll
";.+ .rJc ,-irJiill ,,,t1 ..{/
( t !:u't r ,1 : Primary culture qlsyr t:l;^ll * 4 - f,/
'"'-i lÂJusl J e-Jl er" !)l-i.ll J.-s +J-s ,rS Jl +l':jl o].!1 Primary çu|{u1s G-r,i
.:+l uÉ ,rk ) il L \ii",Jl 4JsL11 â=.l ,,JI Js +Ul l).=il ,-§i:: el Jl à-i) ai+ .jr15
,",-r'-l l+),tJl 9l .,-:43 cOnfluent stage ç .-,§! L Jl J.:: !)\ilt Jd .1l gl ,(g-p::l
t-!!tl d subculture l+)iÀll Li : rJ.-Â;:!cl cll. ".+,.,îi .i,"à*.,11 1{+àr 6^ â--:"^ J;.-Ldi^
. cell passage .r 1I"Jl .)À .,lc §.IL .J Ili JIJ,"iYl ,1- "S;:l
i+.:+ &,rjÀl-Jl .,J- -;;L
: Cell tine r+)till.Li ,t,3/
cell line !)i li .+ primary culture ql-,Yl t-ll:Jl ,.--,: ,11):JJ subculture ql^c J3t :-r
finite Ërr- r ËL»' i;;r rili U-:Yl t-lly.ll d âiii.j:^il $Jlllt= .:,i .subclone i+rj il+.,i _rl
r JÀil J' +J1Jt ôJi.ill ôi: !}ÀJl g+.-j passage !)liJt J"-i Âjl^Ç JIJ,^:-L . iife span
L^1Jl lr)-ill c.o5+i ,P âo'r^ !-Bl^ cJÀ"-
-,
jlr.-r jlJÀ li j^.r 4iÊ ë:-ir 1". ;sl-..Jl r al.,âty1
. cell population
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: Cell strain L.JÈi a§ * 3 -- C"
.ç>l o+-rh ,i ;t..,ï.,')l â1"-1:» çrl J= !À 4r jr ; la)i .'Liül .;^ 3,Hl +9lJlJ.=lf 3o
,i ;:=1, {iJ"S ,a: ;i tpti)t "l+l üS"J !}Àll "iÀ .5-à Jêlü {!" .:l_.;^^ _,
i:i.i.o ,.ti .+ t r
!il.S
1*)lill (§tS ljt Homogeneous population L-,iÉJ! .-,,Jl ,É ÇUltacr^-," ^; *
t.':,J1 â;'i:. à-,iJt .l
d !)tJt c,l§ lil [Ieterogeneous population 4§ljjJl ."s q)À]l i-er,"=. **; *
L:ut ;ru^ L_,_,Jl
ài.rll"ü^ 11}.--l! "ia ü:Si ,Jt-:lt+ _e â-:s.13 Êi 4;li i,,^ i..,L;,: i.'oJl â;t.:^ 1{)À 4.cJ^-^ Clone *
.S1.ly
:continuous cell line J,âill i.ri.,,.ll ; linite ,9.crll ôl3.rrn tJ,)lÀll LJLI. * tl-r2,
c-rLliüYl ô.§ rrs^ tlr it .,.iiii çl 19l) fL"nYl : :^Jl ,rlg;:rt-o;;$ â-r,rtll 19)ÀJl ,;1h.:
4À È""jill àLj Jl J.".i : pliitYl .rlc. ;;:!ilt 4"'s .rÂi: u1,-" ;*e Jl Ji^-l ül Jl (oi,rtrll
J.Jti LÈ,i C+l :à ,éll.: dJ- Éjl ,,Jc . tinite cel| line+ !)L=Jl o:a -lL."-i .senescence
ô1^a+ crÀi rÀ é1; t.unrformation #l:_dt 1.j-9.:-iil i+J^c. a;1, immortal Ë;,anx U)Àll
continuou5 i;,ei-'e U)ÀllJ,lLi' e+,.-l .4iF- aib^§ rl +;-»Li é>t ptsi[ ,n,-i,J Jl 4$ii
. )"iJl tUüYl 4j k+*l.t é1 _r,ft;_Jt J_rJl i.r'J.c u., cell lines
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