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3.3. Determination of Physicochemical Parameters
Five-day biochemical oxygen demand (BOD5) was measured by means of a WTW OxiTop OC 110 system and a WTW TS 606-G/2-i thermostat cabinet (WTW, Weilheim, Ger- many). The chemical oxygen demand (COD) was determined using a WTW Thermoreactor 3200 and a WTW PHotoFlex portable photometer, following the corresponding set of test
WTW, Weilheim, Germany). Inorganic ions were determined using an ion chromatograph (Shimadzu CDD-6A) equipped with a column (Shimadzu Shim-pack IC-A3 150 x 4.00 mm, 5 μm), a column oven (CTO-10A VP) and a conductivity detector (Shimadzu CBM-20A). The mobile phase consisted of 50 mmol boric acid, 3.2 mmol/L BisTris and 8 mmol/L p-hydroxy benzoic acid, flowing at a rate of 1.1 mL min-1. The temperature was set at 40 °C. The volume injected onto the chromatograph was 50 μL. The total phenolic content of the samples was determined using the Folin-Ciocalteau (FC) colorimetric assay. A 5 mL portion of the sample was mixed with 250 µL of FC reagent. After 2 min, 750 µL of Na2CO3 solution was added. After 1 h of standing in dark, the absorbance was measured at a wavelength of 750 nm using a UV-Vis spectrophotometer (Jasco-V630, Tokyo, Japan). Absorbance at 254 nm was determined using a UV-Vis spectrophotometer (Jasco-V630, Tokyo, Japan) in order to indirectly evaluate the aromatic compound content. Fluores- cence emission-excitation matrices (EEMS) of the secondary and photocatalytically treated wastewaters were measured using a Fluoromax+ (Horiba) luminescence spectrometer, by means of scanning the emission wavelengths (Em) from 260 to 600 nm at 1 nm-increments and stepping through the excitation wavelengths (Ex) from 240 to 550 nm at 5 nm intervals. Excitation and emission slits were both adjusted to 1 nm.
3.3. Determination of Physicochemical Parameters
Five-day biochemical oxygen demand (BOD5) was measured by means of a WTW OxiTop OC 110 system and a WTW TS 606-G/2-i thermostat cabinet (WTW, Weilheim, Ger- many). The chemical oxygen demand (COD) was determined using a WTW Thermoreactor 3200 and a WTW PHotoFlex portable photometer, following the corresponding set of test
WTW, Weilheim, Germany). Inorganic ions were determined using an ion chromatograph (Shimadzu CDD-6A) equipped with a column (Shimadzu Shim-pack IC-A3 150 x 4.00 mm, 5 μm), a column oven (CTO-10A VP) and a conductivity detector (Shimadzu CBM-20A). The mobile phase consisted of 50 mmol boric acid, 3.2 mmol/L BisTris and 8 mmol/L p-hydroxy benzoic acid, flowing at a rate of 1.1 mL min-1. The temperature was set at 40 °C. The volume injected onto the chromatograph was 50 μL. The total phenolic content of the samples was determined using the Folin-Ciocalteau (FC) colorimetric assay. A 5 mL portion of the sample was mixed with 250 µL of FC reagent. After 2 min, 750 µL of Na2CO3 solution was added. After 1 h of standing in dark, the absorbance was measured at a wavelength of 750 nm using a UV-Vis spectrophotometer (Jasco-V630, Tokyo, Japan). Absorbance at 254 nm was determined using a UV-Vis spectrophotometer (Jasco-V630, Tokyo, Japan) in order to indirectly evaluate the aromatic compound content. Fluores- cence emission-excitation matrices (EEMS) of the secondary and photocatalytically treated wastewaters were measured using a Fluoromax+ (Horiba) luminescence spectrometer, by means of scanning the emission wavelengths (Em) from 260 to 600 nm at 1 nm-increments and stepping through the excitation wavelengths (Ex) from 240 to 550 nm at 5 nm intervals. Excitation and emission slits were both adjusted to 1 nm.
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