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On the basis of morphological, biochemical, 16S rRNA gene sequencing and phylogeny analysis, the isolates were authentically identified as Shewanella oneidensis in addition to Bacillus thuringiensis and Deinococcus radiodurans.This can be clarified from an elevated percentage of Pb through absorbed (51.06%) in 2h and the absorption of Cd (63.46%) in concentration 50 mgL1.These bacteria were isolated from soil collected from Al-Zubair district in Basra governorate southern Iraq.The minimal inhibitory concentration (MIC) of isolates against cadmium (Cd) and lead (Pb) was determined on solid medium.
On the basis of morphological, biochemical, 16S rRNA gene sequencing and phylogeny analysis, the isolates were authentically identified as Shewanella oneidensis in addition to Bacillus thuringiensis and Deinococcus radiodurans. These bacteria were isolated from soil collected from Al-Zubair district in Basra governorate southern Iraq. The minimal inhibitory concentration (MIC) of isolates against cadmium (Cd) and lead (Pb) was determined on solid medium. S oneidensis showed signiûcant resistance to high concentrations of Cd (1000 mgL¹) and Pb (700mgL¹). The bioasorption capabilities of S. oneidensis for Cd and Pb were monitored at different ion concentrations and contact times. The results of biosorption experiment showed that S. oneidensis has a good ability to absorb both Cd and Pb in considering to the metals concentrations and times. This can be clarified from an elevated percentage of Pb through absorbed (51.06%) in 2h and the absorption of Cd (63.46%) in concentration 50 mgL¹.
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